primary antibody anti p2y 6 r  (Alomone Labs)

Journal: Purinergic Signalling

Article Title: Proteasomal-dependent endothelial P2Y 6 receptor downregulation as an adaptive mechanism limiting monocyte adhesion during intestinal schistosomiasis

doi: 10.1007/s11302-026-10141-x

Figure Lengend Snippet: Endothelial P2Y 6 R mRNA and protein levels are reduced during schistosomiasis. A. Immunocytochemistry staining of cultured mesenteric endothelial cells from control (top line) and infected (bottom line) groups using anti-P2Y 6 R antibody (1:500, overnight), followed by Alexa Fluor 488 secondary antibody (1:300, 1 h, green) and nuclear fluorescence using DAPI (blue) (scale bar = 20 μm; × 400) (see methods). B. The fluorescence intensity was quantified and then expressed as arbitrary units (a.u.). P2Y 6 R mRNA levels were lower in the infected (gray bars) than in the control (white bars) group. C. The P2Y 6 R mRNA levels were normalized by the endogenous gene β-actin. Data were expressed as mean and SEM of n independent cultures performed with different animals for each condition. ***p < 0.001, **p < 0.01 (unpaired t -test, n = 3–4 animals for each group)

Article Snippet: Primary antibody anti-P2Y 6 R (#APR-011) was obtained from Alomone Labs.

Techniques: Immunocytochemistry, Staining, Cell Culture, Control, Infection, Fluorescence

Journal: Purinergic Signalling

Article Title: Proteasomal-dependent endothelial P2Y 6 receptor downregulation as an adaptive mechanism limiting monocyte adhesion during intestinal schistosomiasis

doi: 10.1007/s11302-026-10141-x

Figure Lengend Snippet: Endothelial P2Y 6 R expression is increased by proteosome inhibition during schistosomiasis. A. Immunocytochemistry staining of cultured mesenteric endothelial cells from basal (top line) MG132 1 µM, 1 h (middle line) or MG132 1 µM, 6 h (bottom line) using anti-P2Y 6 R antibody (1:500, overnight) followed by Alexa Fluor 488 secondary antibody secondary antibody (1:300, 1 h, green) and nuclear fluorescence using DAPI (blue) (scale bar = 20 μm; × 400) (see methods). B. The fluorescence intensity was quantified for all groups and then expressed as arbitrary units (a.u.). C . ROS was quantified colorimetrically using NBT. D . Lipid peroxidation was quantified by the production of malondialdehyde (MDA) in reaction with thiobarbituric acid. Data were expressed as the mean and SEM of n independent cultures in triplicate for each condition. **p < 0.01 ( B , One-way ANOVA followed by Tukey’s multiple comparisons test, n = 3 animals for each group); ***p < 0.001; **p < 0.01 (C, D, unpaired t -test, n = 3 animals for each group). ROS = reactive oxygen species; TBARS = thiobarbituric acid reactive species

Article Snippet: Primary antibody anti-P2Y 6 R (#APR-011) was obtained from Alomone Labs.

Techniques: Expressing, Inhibition, Immunocytochemistry, Staining, Cell Culture, Fluorescence

Journal: PLoS ONE

Article Title: Enhancement of Glucose Uptake in Mouse Skeletal Muscle Cells and Adipocytes by P2Y 6 Receptor Agonists

doi: 10.1371/journal.pone.0116203

Figure Lengend Snippet: Structures of P2Y 6 R ligands: nucleotide agonists.

Article Snippet: P2Y 6 R specific antibody was from Alomone Labs Ltd. (Jerusalem, Israel).

Techniques:

Journal: PLoS ONE

Article Title: Enhancement of Glucose Uptake in Mouse Skeletal Muscle Cells and Adipocytes by P2Y 6 Receptor Agonists

doi: 10.1371/journal.pone.0116203

Figure Lengend Snippet: MRS2957 was applied in the presence or absence of P2Y 6 R antagonist MRS2578 (1 µM). * P <0.05, when compared to controls (n = 3).

Article Snippet: P2Y 6 R specific antibody was from Alomone Labs Ltd. (Jerusalem, Israel).

Techniques:

Journal: PLoS ONE

Article Title: Enhancement of Glucose Uptake in Mouse Skeletal Muscle Cells and Adipocytes by P2Y 6 Receptor Agonists

doi: 10.1371/journal.pone.0116203

Figure Lengend Snippet: C2C12 cells (A) or 3T3-L1 cells (B) were preincubated with inhibitors of MAPK (U0126, 10 µM) and AMPK (Compound C, 10 µM) followed by treatment with P2Y 6 R agonist MRS2957. * P <0.05, when compared to controls, # P <0.05, when compared to MRS2957, 100 nM (n = 3).

Article Snippet: P2Y 6 R specific antibody was from Alomone Labs Ltd. (Jerusalem, Israel).

Techniques:

Journal: Physiological Reports

Article Title: Changes in P2Y 6 receptor‐mediated vasoreactivity following focal and global ischemia

doi: 10.14814/phy2.15283

Figure Lengend Snippet: Confocal microscope images demonstrating expression of P2Y 6 (FITC, green) in cross‐sections of middle cerebral arteries. DAPI (4′,6‐Diamidine‐2′‐phenylindole dihydrochloride, a blue nucleus stain, has been added. The tMCAO, Sham, and SAH images were generated by superimposing a DAPI stained image on a FITC stained image. The negative control demonstrates the lack of unspecific antibody binding and the autofluorescence of the lamina elastica interna which separates the intima from the underlying layer of smooth muscle cells

Article Snippet: The cell suspensions were stained overnight at +4°C temperature with primary goat anti‐SM22α (1:100, Abcam) and primary rabbit anti‐P2Y 6 (1:100, Alomone), respectively or goat isotope control IgG (Abcam), rabbit isotope control IgG (Abcam) in blocking buffer (PBS + 5% normal donkey serum) protected from light with rocking, respectively.

Techniques: Microscopy, Expressing, Staining, Generated, Negative Control, Binding Assay

Journal: Physiological Reports

Article Title: Changes in P2Y 6 receptor‐mediated vasoreactivity following focal and global ischemia

doi: 10.14814/phy2.15283

Figure Lengend Snippet: Bar graph with scatter plot demonstrating the expression of P2Y 6 receptors on vascular smooth muscle cells determined by flow cytometry. Data are presented as median and IQR, n = 3–6, * p < 0.05

Article Snippet: The cell suspensions were stained overnight at +4°C temperature with primary goat anti‐SM22α (1:100, Abcam) and primary rabbit anti‐P2Y 6 (1:100, Alomone), respectively or goat isotope control IgG (Abcam), rabbit isotope control IgG (Abcam) in blocking buffer (PBS + 5% normal donkey serum) protected from light with rocking, respectively.

Techniques: Expressing, Flow Cytometry

Journal: Physiological Reports

Article Title: Changes in P2Y 6 receptor‐mediated vasoreactivity following focal and global ischemia

doi: 10.14814/phy2.15283

Figure Lengend Snippet: Representative dot plots and histograms of the experimental setup and analysis of flow cytometry data. (a) Dot plot histogram for entire cell population after isolation. (b) Dot plot histogram of single cells population according to FSC‐H (H—high) versus FSC‐A (A—area scaling). (c) Representative histogram of negative single cells population for Fixable Viability Dye eFluor 780 (viable cells, live/dead); (d) Representative histogram demonstrating SM22α‐positive events of viable cells suspension (log scale). (e) Representative histograms demonstrating P2Y 6 ‐positive events of viable VSMC suspension (log scale)

Article Snippet: The cell suspensions were stained overnight at +4°C temperature with primary goat anti‐SM22α (1:100, Abcam) and primary rabbit anti‐P2Y 6 (1:100, Alomone), respectively or goat isotope control IgG (Abcam), rabbit isotope control IgG (Abcam) in blocking buffer (PBS + 5% normal donkey serum) protected from light with rocking, respectively.

Techniques: Flow Cytometry, Isolation